Expression values of specific genes (in technical replicates) are represented by colour intensities as shown in the reference colour key. ( c) Hierarchical clustering of the differentially expressed genes depicting 4 high altitude time points as compared with control. Overlap is shown among and between all high altitude time points.
#TOPOLOGICAL FEATURES USING CYTOSCAPE 3.2.1 SERIES#
( a) Differentially expressed genes represented as bar graph–comparative up regulated and down regulated bars of all high altitude time points in comparison to basal ( b) Venn diagram show the overlap of high altitude time series DEGs in comparison to basal. Abbreviations: HAD3, high altitude day 3 HAD7, high altitude day 7 HAD14, high altitude day 14 HA21, high altitude day 21 BP-sys, systolic blood pressure BP-dia, diastolic blood pressure mPAP, mean pulmonary artery pressure AMS, Acute Mountain Sickness. ( f) Represents the AMS scoring obtained at first 7 high altitude days of 30 individuals. Statistical significance is represented as *p<0.05, **p<0.01, and ***p<0.001 at different high altitude time points in comparison to basal. Line graph showing changes mean±SEM of 30 individuals at basal and different high altitude time points HAD3, HAD7, HAD14, and HAD21 for ( a-e) BP-sys, BP-dia, Heart Rate, Arterial oxygen saturation, and mPAP. It includes: Sample collection, data collection, pre-processing, analysis and validation of the data.
Schematic representation of the work flow approach undertaken for the high altitude temporal analysis. Primers designed used for technical validation of gene expression by real time PCR.
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